Peptide Reconstitution Calculator
Calculate the correct solvent volume, concentration, and injection dosing for any research peptide.
Input Values
The total amount of peptide in the vial, printed on the label
BAC water for most peptides, PBS for GHK-Cu
1mg = 1000mcg
Results
Concentration After Reconstitution
2500 mcg/mL
(2.50 mg/mL)
Injection Volume Per Dose
0.100 mL
(10.0 units on U-100 syringe)
Doses Per Vial
20 doses
at 250 mcg per dose from a 5mg vial
Total Peptide in Vial
5mg = 5,000 mcg
Quick Reference: Common Peptide Dosing
| Peptide | Typical Dose | Frequency | Solvent |
|---|---|---|---|
| BPC-157 | 250-500 mcg | 2x daily | BAC Water |
| TB-500 | 2-5 mg/week | 2x weekly | BAC Water |
| GHK-Cu | 2 mg | 5 on / 2 off | PBS |
| Semaglutide | 0.25-2.4 mg | 1x weekly | BAC Water |
| Tirzepatide | 2.5-15 mg | 1x weekly | BAC Water |
| CJC-1295 (no DAC) | 100-200 mcg | 1x daily (before bed) | BAC Water |
| Ipamorelin | 200-300 mcg | 1x daily (before bed) | BAC Water |
Dosing information is compiled from community research protocols and published studies. This is for reference only — always consult applicable research literature.
Frequently Asked Questions
How do I calculate peptide reconstitution volume?
Divide the vial size in mg by your desired concentration in mg/mL to get the solvent volume. For example, a 5mg vial with 2mL of BAC water gives a concentration of 2.5mg/mL (2,500mcg/mL).
How many units on a U-100 syringe equals 1mL?
A U-100 insulin syringe has 100 units per 1mL. So 10 units = 0.1mL, 25 units = 0.25mL, and 50 units = 0.5mL.
Should I use BAC water or PBS?
Most peptides use bacteriostatic water (BAC water) with 0.9% benzyl alcohol. GHK-Cu is the main exception — it requires Phosphate Buffered Saline (PBS) for the correct pH environment. See our GHK-Cu guide for details.
How long does a reconstituted peptide last?
With BAC water, refrigerated at 2-8°C, most peptides last approximately 30 days. GHK-Cu in PBS (without preservative) should be used within 1-2 days. See our Peptide Storage Guide for full details.
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